This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
1. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs
Stella Diafa, Damien Evéquoz, Christian J. Leumann, and Marcel Hollenstein
2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine
Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows
3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue
Hui Mei and John Chaput
4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA
Petra Ménová, Hana Cahová, Milan Vrábel, and Michal Hocek
5. 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability
Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari
6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids
Tanaya Bose and Vaijayanti A. Kumar
7. Synthesis of Nucleobase-Functionalized Morpholino Monomers
Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha
8. Synthesis and Application of LKγT Peptide Nucleic Acids
Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella
9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids
Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya
10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site
Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates
11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids
Malte Rosenthal, Franziska Pfeiffer, and Günter Mayer
12. Labeling Peptide Nucleic Acids with Indium-111
Igor G. Panyutin
13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet
Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg
14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates
Maryline Chemama and Christopher Switzer
15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis
Jung-Suk Choi and Anthony Berdis
16. FRET Assay for Ligands Targeting the Bacterial A-Site RNA
Renatus W. Sinkeldam and Yitzhak Tor
17. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons
Keiji Murayama, Hiromu Kashida, and Hiroyuki Asanuma
18. Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA
Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, and Andrey V. Aralov
19. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation
Hailey L. Gahlon and Shana J. Sturla
This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Includes cutting-edge techniques
