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Tumor Immunology

Methods and Protocols

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EAN: N/A SKU: 9781493980246 Categories: ,

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Weight 354 g
Dimensions 178 × 254 mm
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About The Author

Bondanza, Attilio

This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories.  This book provides a better understanding of the rules governing tumor and cancer immunology, and discusses innovations in the technology of the immunological “smart bullets” (monoclonal antibodies, vaccines, tumor-reactive T cells) used to specifically target cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Tumor Immunology: Methods and Protocols contains a wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

1. 1 1. ATMPs for Cancer Immunotherapy: A Regulatory Overview
Maria Cristina Galli

2. Natural Antibodies to Tumor-Associated Antigens
Sheila Maria Álvarez-Fernández, Lucia De Monte, and Massimo Alessio

3. Generation and Cryopreservation of Clinical Grade Wilms’ Tumor 1 mRNA-Loaded Dendritic Cell Vaccines for Cancer Immunotherapy
Evelien L.J.M. Smits, Barbara Stein, Viggo F. Van Tendeloo, Yannick Willemen, Sébastien Anguille, and Zwi N. Berneman

4. Quantitative and Qualitative Analysis of Tumor Associated CD4T Cells
Giulia Di Lullo, Lucia De Monte, Claudio Doglioni, and Maria Pia Protti

5. Detection and Functional Analysis of Tumor-Derived LXR Ligands
Raffaella Fontana, Aida

Paniccia, and Vincenzo Russo

6. In Vitro Generation of Antigen Specific T Cells from Induced Pluripotent Stem Cells of Antigen Specific T Cell Origin<Shin Kaneko

7. Chimeric Antigen Receptors for Cancer Immunotherapy
Claudia Geldres, Barbara Savoldo, and Gianpietro Dotti

8. Cancer and Chemokines
Nicoletta Caronni, Benedetta Savino, Camilla Recordati, Alessandro M.G. Villa, Massimo Locati, and Raffaella Bonecchi

9. T Cells as Antigen-Carriers for Anti-Tumor Vaccination
Catia Traversari and Vincenzo Russo

10. Exploiting Secreted Luciferases to Monitor Tumor Progression In Vivo
Laura Falcone and Monica Casucci

11. Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models
Benedetta Nicolis di Robilant and Maddalena Noviello

12. Modeling Human Graft-Versus-Host-Disease in Immunocompromised Mice
Margherita Norelli, Barbara Camisa, and Attilio Bondanza

13. ICOS Expression as Immunologic Marker in Immune Activating Monoclonal Antibodies
Riccardo Danielli, Ornella Cutaia, Carolin Fazio, Erika Bertocci, Ester Fonsatti. Michele Maio, and Luana Calabrò

14. Identifying NK Alloreactive Donors for Haploidentical Hematopoietic Stem Cell Transplantation
Loredana Ruggeri, Antonella Mancusi, Elena Urbani, and Andrea Velardi

15. Human Microtumors Generated in 3D: Novel Tools for Integrated In Situ Studies of Cancer Immunotherapies
Lothar Hambach, Andreas Buser, Marcel Vermeij, Nadine Pouw, Theo van der Kwast, and Els Goulmy


This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories.  This book provides a better understanding of the rules governing tumor and cancer immunology, and discusses innovations in the technology of the immunological “smart bullets” (monoclonal antibodies, vaccines, tumor-reactive T cells) used to specifically target cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Tumor Immunology: Methods and Protocols contains a wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable. Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts