This volume highlights proteomics studies of quantitative PTM changes in both peripheral and central nervous system proteomes utilizing the most recent advances in mass spectrometry. Chapters include practical information pertaining to the fundamentals of sample preparation, liquid chromatography, and tandem mass spectrometry instrumental analysis and will elucidate best practices in the interpretation of data using modern bioinformatics approaches. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.
Authoritative and practical, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience aims to ensure successful results in the further study of this vital field.
1. Proteomic Analysis of Post-Translational Modifications in Neurobiology
Hongbo Gu, Matthew P. Stokes, and Jeffrey C. Silva
2. A Boronic Acid-based Enrichment for Site-specific Identification of the N-glycoproteome using MS-based Proteomics
Haopeng Xiao, George X. Tang, Weixuan Chen, and Ronghu Wu
3. Site-specific Localization of D-Amino Acids in Bioactive Peptides by Ion Mobility Spectrometry
Chenxi Jia, Christopher B. Lietz, Qing Yu, and Lingjun Li
4. Quantitative Profiling of Reversible Cysteome Modification under Nitrosative Stress
Yue-Ting Wang, Sujeewa C. Piyankarage, and Gregory R. J. Thatcher
5. Label-free LC-MS/MS Comparative Analysis of Protein S-nitrosome in Synaptosomes from Wild Type and APP Transgenic Mice
Monika Zaręba-Kozioł, Maciej Lalowski, and Aleksandra Wysłouch-Cieszyńska
6. Proteomics Identification of Redox-Sensitive Nuclear Protein Targets Of Human Thioredoxin 1 from SHSY-5Y Neuroblastoma Cell Line
Mohit Raja Jain, Changgong Wu, Qing Li, Chuanlong Cui, Huacheng Dai, and Hong Li
7. Identification and Quantification of K63-Ubiquitinated Proteins in Neuronal Cells by Hi
gh-
Resolution Mass Spectrometry
Gustavo Monteiro Silva, Wei Wei, Sandhya Manohar, and Christine Vogel
8. Phosphorylation Site Profiling of NG108 Cells Using Quadrupole/Orbitrap Mass
Spectrometry
Fang-Ke Huang, Guoan Zhang, and Thomas A. Neubert
9. Phosphoproteomics of Tyrosine Kinases in the Nervous System
Robert J. Chalkley and
Ralph A. Bradshaw
10. Analysis of PINK1 and CaMKII Substrates Using Mass Spectrometry-Based Proteomics
Yan Li, Lesley A. Kane, Michael A Bemben, and Katherine W Roche
11. Liquid Chromatography-Tandem Mass Spectrometry Analysis of Tau Phosphorylation
Jhoana Mendoza,Georgia Dolios, and Rong Wang
12. Identification of Protease Substrates in Complex Proteomes by iTRAQ-TAILS
Tobias Kockmann, Nathalie Carte, Samu Melkko, and Ulrich auf dem Keller
13. Proteolytic P
rocessing of Neuropeptides
Lloyd D. Fricker
14 Isotope Dilution Analysis of Myelin Basic Protein Degradation after Brain Injury
Andrew K Ottens
15. Identification and Characterization of Protein Post-Translational Modifications by
Differential Fluorescent Labeling
Eric D. Hamlett, Cristina Osorio, and Oscar Alzate
16 Mass
Spectrometric Detection of Detyrosination and Polyglutamyation on the C-Terminal Region of Brain Tubulin
Yasuko Mori, Alu Konno, Mitsutoshi Setou, and Koji Ikegami
17. Determination of polyhydroxybutyrate (PHB) Post-Translational Modifications of Proteins Using Mass Spectrometry
Tong Liu, Wei Chen, Stacey Pan, Chuanlong Cui, Hong Li, and Eleonora Zakharian
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
