Neurotransmitter Transporters

Investigative Methods

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EAN: N/A SKU: 9781493981328 Category:

Book Details

Weight 470 g
Dimensions 178 × 254 mm


Book Cover

Paperback / softback


Springer New York



Publishing Date


About The Author

Bönisch, Heinz

The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced undergraduates who seek an overview of methods and protocols in this field of neuroscience. It will also be of interest to physicians who are carrying out imaging and postmortem studies of neurotransmitter transporters in the human brain. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the
further study of this vital field.

Part I: Recent Improvements
in LV Construction, Production, and Transduction

1.      Construction of Modular Lentiviral Vectors for Effective Gene
Expression and Knockdown

Angeline De Bruyns, Ben Geilin, and David Dankort


2.      Development of Inducible Molecular Switches Based on All-In-One
Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase

Tobias Maetzig and Axel


Production Of Retrovirus-Based
Vectors In Mildly Acidic Ph Conditions

Nathalie Holic And David Fenard


4.      Optimized Lentiviral
Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and
Scfv-Antibody Fusions To VSV-G

Nataša Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt,
and Christian Thirion


Part II: New LV Targets
and Applications

Transduction of Murine
Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors

Maike Stahlhut, Axel Schambach, and Olga
S. Kustikova


6.      Introduction of Shrnas, Mirnas or Antagomirs into
Primary Human Liver Cells Through Lentiviral Vectors

K. Rieger and Maria Thomas


7.      Production and Concentration of Lentivirus for Transduction of Primary
Human T Cells

Alan Kennedy and Adam P. Cribbs


8.      Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa
Followed by In Vitro Fertilization and Embryo Transfer

Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, and Robert Winston


9.      The LAM-PCR Method to Sequence LV Integration Sites

Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette
Deichmann, and Manfred Schmidt


10.  Conditional Rnai using the Lentiviral GLTR System

Elisabeth Pfeiffenberger and Stephan Geley


11.  Lentiviral Vectors for the Engineering of Implantable Cells Secreting
Recombinant Antibodies

Aurélien Lathuilière and Bernard L. Schneider


III. Integrase-Mutant

12.  Transient Expression of Green Fluorescent Protein in Integrase-Defective
Lentiviral Vector Transduced 293T Cell Line

Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin
Farzaneh, and MK Azaham A Hamid


13.  Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a
Rat Model of Parkinson’s Disease

Ngoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. Yáñez-Muñoz


14.  Development of Lentiviral Vectors for Targeted Integration and Protein

Diana Schenkwein and Seppo Ylä-Herttuala


Part IV: Production,
Detection, and Engineering of Exosomes

15.  Biogenesis and Functions of Exosomes and Extracellular Vesicles

Florian Dreyer and
Andreas Baur


16.  Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes

Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana
Zanetti, Maria Luisa Fiani, and Massimo Sargiacomo


17.  Cardiac Myocyte Exosome Isolation

Zulfiqar A. Malik and Anne A. Knowlton


of Heterologous Proteins in Engineered Exosomes

Francesco Manfredi, Paola Di
Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico


19.  Exosome-Mediated Targeted Delivery of

Ohno and Masahiko Kuroda





Includes cutting-edge methods and protocols 

Provides step-by-step detail essential for reproducible results 
Contains key notes and implementation advice from the experts