This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
1. CD36 Neuronal Identity in the Olfactory Epithelium
André Machado Xavier and Isaias Glezer
2. Deorphanization of Olfactory Trace Amine-Associated Receptors
Qian Li
3. G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction
Sayoko Ihara and Kazushige Touhara
4. Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging
Pablo Chamero and Frank Zufall
5. Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates
Chunbo Zhang
6. Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations
Artur G. Leme Silva, Maíra H. Nagai, and Bettina Malnic
7. Numerical Models and In Vitro Assays to Study Odorant Receptors
Caroline Bushdid, Claire A. de March, Hiroaki Matsunami, and Jérôme Golebiowski
8. High-Throughput Odorant Receptor Deorphanization Via pS6-Immunoprecipitation and mRNA Profiling
Xiaoyang Serene Hu and Hiroaki Matsunami
9. Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons
Anna Boccaccio
10. In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-Olfactograms in Anesthetized Rats
Patricia Duchamp-Viret and Michel Chaput
11. Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction
Michele Dibattista and Johannes Reisert
12. Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ
Xavier Grosmaitre and Minghong Ma
13. Long-term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multi-Electrode in Acute Brain Slice Preparations
Michael Lukas, Knut Holthoff, and Veronica Egger
14. Multi-Site Recording of Local Field Potentials in Awake, Free-Moving Mice
Xingfeng Mao, Tiantian Cao, and Anan Li
15. In Vivo Two-Photon Imaging of the Olfactory System in Insects
Marco Paoli and Albrecht Haase
16. Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder
Hirokazu Kumazaki, Masako Okamoto, Syo Kanzaki, Ken-ichi Okada, Masaru Mimura, Yoshio Minabe, and Mitsuru Kikuchi
17. Methods in Rodent Chemosensory Cognition
Alan Gelperin
18. Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs
Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, and Tai Hyun Park
19. Tracking Odorant Plumes
Matthew A. Reidenbach
20. Generative Biophysical Modeling of Dynamical Networks in the Olfactory System
Guoshi Li and Thomas A. Cleland
21. Behavioral Assays in the Study of Olfaction: A Practical Guide
Fabio Papes, Thiago S. Nakahara, and Antonio P. Camargo
This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Includes cutting-edge techniques for the study of olfactory receptors
